GelRed Nucleic Acid Gel Stain Higher Sensitivity than EB Safe and Non-Mutagenic

Product Information：

GelRed is a highly sensitive, non-mutagenic, ultra-safe and ultra-stable fluorescent nucleic acid gel staining reagent (working concentration), which can replace ethidium bromide (EtBr, EB) with much higher sensitivity than EB without decolorization. GelRed has the same spectral characteristics as EB, and replaces EB without changing the imaging system.

Storage Conditions：

Ship and store at room temperature; valid for 24 months.

Instructions for Use:

1. Glue dyeing method (same usage as EB)
a. To prepare the gel, add 5 μL of GelRed Nucleic Acid Dye (10,000×, water soluble) per 50 mL of agarose (YB-AG-100G recommended) gel and mix thoroughly (GelRed has excellent thermal stability and can be added directly to the high-temperature gel solution without waiting for the gel solution to cool. It can also be prepared by pre-mixing GelRed with an electrophoresis buffer containing agarose powder and heating it).
b. Electrophoresis according to conventional methods.

2. Soak dyeing method
a. Electrophoresis according to conventional methods.
b. Dilute GelRed Nucleic Acid Dye 10,000× stock solution approximately 3,300-fold dilution to make a 3× staining solution (for example, add 15 μL of GelRed Nucleic Acid Dye 10,000× stock solution to 50 mL of H2O).
c. Carefully place the gel into a suitable container and soak the gel with a sufficient amount of 3× staining solution. In order to shorten the soaking time, the dyeing solution can be pre-heated to about 70℃, then put into the gel and incubate for 10 minutes to obtain the ideal effect (If not heated, incubate for 30 minutes in room temperature shaker; if it is acrylamide gel, incubate for 30-60 minutes and extend with the increase of acrylamide content). The amount of bubble dyeing dye is large, and the dyeing solution can be reused for about 3 times for a single use. The 3× GelRed stain solution can be prepared in large quantities and stored at room temperature until used up.

Notice: 

a. The dye does not need to be refrigerated at low temperature. Please store it at room temperature to avoid precipitation. If precipitation is found, please heat the dyes to 45-50°C for 2 minutes and shake to dissolve, which does not affect the use.
b. This product can stain single-stranded DNA and RNA, but is less sensitive to single-stranded DNA or RNA than double-stranded DNA.
c. Wear lab coats and disposable gloves during operation.
For research Use Only