Accurate protein quantification is essential in biochemistry and life science research. Whether you are preparing samples for electrophoresis, immunoassays, or cell biology experiments, reliable protein concentration determination ensures reproducibility and confidence in your results.
At Yanbiotech, we offer two widely used protein quantification methods: Bradford and BCA. Each has distinct advantages depending on your sample composition and experimental needs.
Product Overview
| Cat. No. | Product Name |
| Y20121-250ML | Bradford protein concentration determination kit |
| Y20122-200T | BCA protein concentration determination kit |
Method Comparison
| Feature | ||
| Detection Range | 100–1000 µg/µL | 25–1000 µg/µL |
| Detergent Compatibility | SDS <0.01%; Triton X-100 <0.05%; Tween <0.015% | SDS ≤5%; Triton X-100 ≤5%; Tween ≤5% |
| Reducing Agent Compatibility | EDTA ≤100 mM; DTT ≤5 mM; 2-ME ≤7.8% | EDTA ≤10 mM; DTT ≤1 mM; 2-ME ≤0.01% |
| Key Advantage | Single reagent, no premixing; room temperature incubation for 5 min | High accuracy, broad linear range, minimal protein-to-protein variation |
Which Method Should You Choose?
Bradford Assay ()
This classic method is known for its speed and simplicity. With only one reagent and a 5-minute incubation at room temperature, it is ideal when detergents and reducing agents are not present in your sample.
Bradford Assay – Detergent Compatible ()
An upgrade to the classic method, this version tolerates moderate levels of detergents (SDS, Triton X-100, Tween up to 1%), making it suitable for most routine cell and tissue lysates. The color signal remains stable for over one hour, providing flexibility during measurement.
BCA Assay ()
If your samples contain detergents or require high accuracy across a broad concentration range, the BCA method is an excellent choice. It offers minimal protein-to-protein variation and a wide linear range. However, it requires a 30-minute incubation at 37°C and is incompatible with reducing agents such as DTT and 2-ME.
Technical Tips
Bradford is fast and convenient, but its standard curve is slightly less linear than BCA, and results may vary between different protein types.
BCA provides greater accuracy and consistency across different proteins, but the assay cannot be used in the presence of reducing agents due to its copper-based detection mechanism.
Conclusion
Selecting the right protein quantification method depends on your sample composition, throughput needs, and compatibility requirements. Meilunbio® offers reliable, ready-to-use kits for both Bradford and BCA methods to support your research with consistency and ease.
For more product details, please refer to our product catalog or contact our technical support team.
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